Closed Solicitation · DEPARTMENT OF HEALTH AND HUMAN SERVICES
AI Summary
The Department of Health and Human Services seeks a vendor to conduct next generation sequencing on DNA samples from CRISPR KO screens. The selected vendor will provide gene counts to analyze gene regulation in colorectal cancer cells. The project requires 6-8 weeks for amplification, sequencing, and reporting of results.
The purpose of this procurement is to use Cellecta’ s Next Generation Sequencing (NGS) Services to sequence DNA samples produced as the result of CRISPR screens, for the Functional Genomics Lab. We require Cellecta to conduct NGS on the submitted samples and provide us with gene counts to determine which genes regulate the survival of wild type versus topoisomerase depleted colorectal cancer cells to elucidate mechanisms of resistance.
NCATS will provide Vendor with 15 extracted gDNA samples from our genome-wide CRISPR KO Screen and Cellecta will then amplify and sequence that DNA by NGS with an average depth of ~500 reads/guide sgRNA distributions from genomic DNA isolated from each sample and the deliverable will consist of a spreadsheet report with sgRNA read counts.
Sequencing technicians will require 6-8 weeks to amplify DNA, sequence it and for their bioinformatician to report the sequencing counts
GENOME WIDE FUNCTIONAL GENOMICS CRISPR KO SCREENING TECHNIQUES is a federal acquisition solicitation issued by DEPARTMENT OF HEALTH AND HUMAN SERVICES. Review the full description, attachments, and submission requirements on SamSearch before the response deadline.
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