PRIMERS AND PROBES

NAMRU SOUTH requests approval to award the purchase of primers and probes for protocol “The Global Travelers Diarrhea (GTD) Study: an evaluation of study design and laboratory methodologies standardization in a multisite protocol assessing the epidemiology and etiology of acquired diarrhea among a U.S. military or civilian travelers.

3. Description of Supplies or Services:
2 units HLT-F– TTC CCA CCG GAT CAC CAA 
2 units HLT-R– CAA CCT TGT GGT GCA TGA TGA
2 units LT-pro – 5 ATTO550N/CTT GGA GAG AAG AAC CCT /MGBNFQ
2 units STh -F– GCTAAACCAGYAGRGTCTTCAAAA
2 units STh -R– CCCGGTACARGCAGGATTACAACA
2 units STh -pro – 5SUN-TGGTCCTGAAAGCATGAA-3MGBMGBNFQ
2 units STp -F – TGAATCACTTGACTCTTCAAAA
2 units STp -R – GGCAGGATTACAACAAAGTT
2 units STp -pro – 56FAM-TGAACAACACATTTTACTGCT-3MGB-NFQ
2 units virB-F – GGA TTT GTG CAA CGA CTT GTT AAG
2 units virB-R– GAG GAA TCT TGG CTT TGA TAA AGG
2 units virB-pro – /56FAM/ CAC TCC ATT CTG GTA ATA A /MGBNFQ/
2 units aggR-333F– CAG CGA TAC ATT AAG ACG CCT AAA G
2 units aggR-448 R– CGT CAG CAT CAG CTA CAA TTA TTC C
2 units aggR-pro – /FAM/ CTT GCA GTT GTC CGA ATT /MGBNFQ/
2 units eae-F– CCG ATT CCT CTG GTG ACG A
2 units eae-R– CCA CGG TTT ATC AAA CTG ATA ACG
2 units eae-pro –5 ATTO550N/iCY3/ CGT CAT GGT ACG GGT AA /MGBNFQ/
4 units bfpA-Fw -GGT CTG TCT TTG ATT GAA TCT GCA
4 units bfpA-Rv-GCA GAC TGG TAG TAA AAC ATC ACA CC
4 units bfpA-pro-/TexasRed/ GCG CTT GCT GCC ACC GTT ACC G /3BHQ_2/
2 units Stx1-414 F– ACT TCT CGA CTG CAA AGA CGT ATG
2 units Stx1-545 R – ACA AAT TAT CCC CTG AGC CAC TAT C
2 units Stx1-pro – /56FAM/ CTC TGC AAT AGG TAC TCC A /MGBNFQ/
2 units HStx2-F– CCA CAT CGG TGT CTG TTA TTA ACC
2 units HStx2-R – GGT CAA AAC GCG CCT GAT AG
2 units Stx2-pro - /5SUN/ TTG CTG TGG ATA TAC GAG G /3MGBNFQ/
4 units GFP-F-AGA TGA CGG GAA CTA CAA G
4 units GFP-Rv-CCT TCA GCT CGA TTC TAT T
4 units GFP-pro-/5Cy5/CA CCT TCG AAC TTG ACT TCA GCG C/3IAbRQSp/
4 units Blocks Gene fragment-TTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATCTTTCAAAGATGACGGGAaCTACAAGACA CGcGCTGAAGTCAAGTTcGAAGGTGATACCCTTGTTAATAGAATCGAGcTgAAgGGTATTGATTTTAAAGAAGATGGA AACATTCTTGGACACAAATTGGAATACAACTTTAACTCACACAATGTATACATCACGGCAGACA
2 units lacY-F- ACCAGACCCAGCACCAGATAAG
2 units lacY-R -CTGCTTCTTTAAGCAACTGGCGA
2 units lacY-DLO-RFB-5 (3)-pro-FAM/CA TAC ATA TTG CCC GCC AGT ACA GAC /3BHQ_1 (3)
2 units uidA-F-GTGTGATATCTACCCGCTTCGC
2 units uidA-R - AGAACGGTTTGTGGTTAATCAGGA
2 units uidA-DLO-HB1-5-pro-HEX/TC GGC ATC CGG TCA GTG GCA GT/3BHQ_1
2 units rfc-F – ACTTATCATTGACTAGCGAGTTCT
2 units rfc-R – CGCAAGCACTATTATGAGACATTT
2 units rfc-DLO-RFB-5-pro-FAM/TG GTG TCG GTG CGA TTA TCA GTT T/3BHQ1/
2 units wbgZ-F – CAGCGTCAGAATCGCTTACA
2 units wbgZ-R – TCAACCAGAGGCACATGTTTAT
2 units wbgZ-DLO-CAB2-5-pro-CAL Fluor Red 610/TT CAA ACG GTT TAT CAT GCG GCC G/3BHQ2/
2 units rfpB-F – ACCAAGAAAGGGATTTCAGTCTAT
2 units rfpB- R – AAGGTTAATACCACTGCGACTT
2 units rfpB-DLO-CGB1-5-CAL Fluor™ Gold 540/AG CAG GCG CCA TTC CTG TTG ATT A/BHQ1
2 units SBO-3648-F– TTGAGCACGGAAACAGAGAG
2 units SBO-3648-R – TTCCCGTTCTTCCCTTTACAC
2 units SBO-3648-DLO-C3B2-5-pro-Cy5/TG CCG CTT CAC ATA AAT CAA CGC C/3BHQ2/
2 units gyrA-F-CTATAACAACTGCACCTACTAAT
2 units gyrA-R-ATGAAATTTTTGCCAGTGGTG
2 units gyrA-pro-FAM/CT TAA TAG CCG TCA CCC CAC /3BHQ_1