REAGENTS FOR SEQUENCING IN MISEQ AND MINISEQ PLATFORMS

PERFORMANCE WORK STATEMENT

1. SCOPE

The laboratory of the Virology Department of the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) is looking to purchase reagents to prepare libraries using human samples that later will be sequenced in the MiSeq and MiniSeq platforms developed by Illumina, as part of the projects of next-generation sequencing (NGS) that are being conducted at this command.

1. 1. BACKGROUND

The kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime ends for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high-quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run.

1. REQUIREMENTS
   1. Location of work / Place of delivery

Shipment to NAMRU SOUTH – Callao, Peru

1. 1. Period of performance / Delivery time

In accordance with deliverables schedule.

1. 1. Deliverables schedule (if applicable)

Materials should be shipped upon award signature.

1. 1. Hazardous material category. 

None of the items are hazardous materials.

List of products

FC-110-3001  PhiX control v3 kit

MS-102-3003  MiSeq Reagent Kit v3 (600-cycle)

FC-131-1096  Nextera XT DNA Sample Preparation Kit

FC-131-2001 Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-420-1003  MiniSeq High Output Reagent Kit (300-cycles)

FC-131-2002  Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

Applicable Standards

These reagents are very time-sensitive and have limited expiration dates, reason for which, vendor should inform final users of expiration dates as soon as they become available and request their approval prior to shipment.

Special Requirements

All materials require cold chain.